Enzymatic Pretreatment Improved the In Vitro Ruminal Degradability of Oil Palm Fronds

This study aims to increase the in vitro ruminal degradability of oil palm fronds (OPFs) through enzymatic pretreatment. The isolated fungi were selected based on their lignocellulosic degrading enzyme activities. Eleven successfully isolated, and activities evaluated. Three fungi, F1, F2 F4 selected, they identified as Trichoderma harzianum MK027305, MK027306 Fusarium solani MK027309, respectively. highest total gas methane production was produced when OPFs pretreated with an extract from 15 30 days solid-state fermentation T. MK027305 MK027306, Meanwhile, F. MK027309 after 45 amount volatile fatty acids. pretreatment using enzymes extracted increases apparent rumen degradable carbohydrate (ARDC) by 35.29% compared unpretreated OPF. showed that fungi’s acid OPF, hence improving livestock via increased utilization agricultural by-products minimal impact cost.